Abstract: In order to eliminate the effect of impurity on the content determination of swainsonine in Oxytropis kansuensis Bunge , macroporous adsorptive resin(MAR) has been used as a sample pretreatment technology. The sample purified with MAR was performed by HPLC and the swainsonine content in Oxytropis kansuensis Bunge was determined. The spiked recovery ratio was 92.81％ with a RSD of 3.67%. RSDs of intra- and inter- day precisions of the method were 1.40% and 3.98%, respectively. The linear range was 0.022~0.730 mg/mL and the detection limit was 0.010 mg/mL. The result showed that the proposed method can remove impurity effectively and is suitable for the content determination of swainsonine in Oxytropis kansuensis Bunge.

Key words: macroporous adsorptive resin; oxytropis kansuensis bunge; swainsonine

Author: DUAN Qiu-yan, 1. Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2. Pharmaceutical School, Lanzhou University, Lanzhou 730000, China

E-mail: didl@lzb.ac.cn　  lynjm2004@hotmail.com

Analysis and Testing Technology and Instruments, Vol. 16, No. 1, 2010, 6-10.