WANG Jian-wei, ZHONG Hai-juan, LIANG Chi-qiong

Foshan Supervision Testing Center of Quality and Metrology, Foshan 528225, China

Abstract: A method for the simultaneous determination of Chrysoidin and Auramine O in food by solid phase extraction-ultra performance liquid chromatography combined with tandem mass spectrometry has been developed. The sample extract was purified by C18 solid-phase extraction column, the analysis can be accomplished within three minutes using this method. Detection limits (S/N=3)of Chrysoidin and Auramine O were 0.34 μg/kg and 1.28 μg/kg, limits of quantification (S/N=10) were 1.13 μg/kg and 4.27 μg/kg, respectively. Recoveries ranged from 80.1% to 95.3%.The method is quick, high sensitive, accurate and reliable and can meet the demands of qualitative and quantitative determinations of low content Chrysoidin and Auramine O in food.

Key words: ultra performance liquid chromatography; tandem mass spectrometry; chrysoidin; auramine O;solid-phase extraction

E-mail: wangjw025@163.com

Analysis and Testing Technology and Instruments, Vol. 16, No. 2, 2010, 108-112.