YAO Chen-wei1, REN Xiao-min2, YAN Zheng-xiong2, HUANG Bao-mei2, BIAN Qing-quan2
(1. Facility Design and Instrumentation Institute of CARDC, Mianyang 622653, China)
2. Chemical Department of Mianyang University, Mianyang 621000, China)
Abstract: Puerarin shows a sensitive derivative oxidation peak at +0.57 V by using the cyclic voltammorgam in buffer solution of 0.02mol/L NH4 Ci-NH3.H2O (pH 8.0). The peak is current is proportional to the concentration of Puerarin in the range of 1.046×10-7～5.767×10-5 mol/L with r=0.9989. The detection limit is 1.046×10-7mol/L. This method has been applied to determine Puerarin with satisfactory results. Its average recovery rate is 99.8%. The electrochemical behavior of the system has been studied at a glassy carbon electrode by cyclic voltammetry. The electrode oxidation of Puerarin at the glassy electrode is irreversible and adsorptive.
Key words: puerarin; cyclic; voltammetry
E-mail: ycwwcy@yahoo.com.cn
Analysis and Testing Technology and Instruments, Vol. 16, No. 2, 2010, 97-99.
