ZHANG Jian-hui1, BAO Chang-li2,YANG Dong-sheng1,LI Jing-ying1
(1.Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai 519041, China;
2. College of Chemistry, Jilin University, Changchun 130012, China)
Abstract: Four ginsenosides, Rg1,Re,Rb1, and Rd of Panax notoginseng were separated and enriched by foam flotation, and high performance liquid chromatography ( HPLC) was used to determine their contents. Effects of concentration and pH of the feed solution, flow rate of nitrogen gas, flotation time and NaCl concentration on the flotation efficiency were investigated. The experimental results showed that high efficiency of separation and enrichment were obtained by foam flotation. The optimum conditions for the foam flotation are feed solution concentration: 2.0 mg/mL, flotation time: 10 min, pH: 2～3, flow rate of nitrogen gas: 20 mL/min and NaCl concentration: 0.20 mol/ L.
Key words: panax notoginseng; ginsenosides; foam flotation; high performance liquid chromatography
E-mail: zjh06@mails.jlu.edu.cn
Analysis and Testing Technology and Instruments, Vol. 16, No. 2, 2010, 89-92.
