LIAO Tao¹, YANG Yu-ping¹, CHENG Wei¹,  XIONG Guang-quan¹, JIN Shi-wei², FANG Ling², GENG Sheng-rong¹, LI Xin¹, LIN Ruo-tai¹, LI Lu¹, YANG Jie³

　  (1. Hubei Innovation Center of Agricultural Science and Technology, Institute for Farm Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; 2 . School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan 430074, China; 3. Food Quality Inspection and Testing Center of Agricultural Ministry of China, Wuhan 430064, China)

Abstract: A high performance liquid chromatographic(HPLC) method has been developed to determine the residues of carbendazim, thiabendazole and thiophanate methyl in apple. Samples were extracted with ethyl acetate, concentrated in a rotary evaporator, purged  by nitrogen, added methanol to a constant volume and determined by HPLC with a diode array detector (DAD). The average spiked recoveries of carbendazim, thiabendazole and thiophanate methyl ranged from 87.7% to 118.7% , 72.8% to 80.3% and 64.0% to 66.8% at different concentrations, and the detection limits of carbendazim, thiabendazole and methylthiophanate in apple samples were 0.134mg/l , 0.230mg/l, and 0.250mg/l, respectively. The results showed the method is suitable for the determination of carbendazim, thiabendazole and methyl thiophanate in apple, and the residueof carbendazim in apple peel was 7.24×10^-2mg/kg, thiabendazole and methyl thiophanate were not detected .These concentrations were lower than the national residual limit standards for carbendazim, thiabendazole and methylthiophanate in apple.

Key words: apple; carbendazim; thiabendazole; thiophanate methyl; HPLC; liquid-liquid extraction

E-mail: xiongguangquan@163.com

Analysis and Testing Technology and Instruments, Vol. 16, No. 4, 2010, 257-261.