NING Sheng-rong1, 2, PEI-Dong2, 3, FENG Zu-fei2, 3, FENG Shi-lan2*, DI Duo-long1,2*
(1. Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 73000, China; 2. School of pharmaceutical, Lanzhou University, Lanzhou, 730000, China; 3. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China)
Abstract: A quality standard for Pule′an Tablet is established. The qualitative identifications of Kaempferol-3-O-β-D-(2-O-β-D-glucosyl)glucopyranoside(KMP) in Pule′an Tablet were performed by TLC; Kaempferol-3,4′-di-O-β-D-glucoside (KMG) and Kaempferol 3-O-β-D-(2-O-β-D-glucosyl)glucopyranoside(KMP) in the formulation were quantified by HPLC. Under optimal conditions, KMG in Pule′an Tablet can be qualitatively and quantitatively identified. Calibration curves of KMG and KMP were found to be linear with in the ranges of 0. 011 2～2.244 0 and 0.011 6～2.236 0 mg/mL, respectively . The correlation coefficient of the calibration curve for the KMG and KMP were 0.999 7 and 0.999 6; the average recoveries were 97.7% and 103.3%, with RSD 1.39% and 1.51%, respectively. This reliable, accurate and specific method can be used for the quality control of Pule'an Tablet.
Key words: Pule'an Tablet; quality standard; TLC; HPLC
E-mail: didl@licp.cas.cn
Analysis and Testing Technology and Instruments, Vol. 17, No. 2, 2011, 83-87.
