Zhong Tian-hua ^[1], Huang Li-ying ^[2], Fang Jing ^[3]

(1. The Third Institute of Oceanography of the State Oceanic Administration, Xiamen 361005; China;2. Fu jian Medical University, Fuzhou 350004,China;3. Tian Jin Institute of Bio-engineering, Tianjin 300462,China)

Abstract: Objective: Objective:The qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Method: The anion chromatography-integral pulse ampere method is used in the qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Results: The limits of detection (IOD) for 17 amino acids fell between 0.23~3.37 pmol, the linearity range of peak area calibration curve (profile) was the order of 2-3 and RSDs for five determinations were 0.96%~4.2%.Conclusion: This method is applicable to the determination of free amino acids in the medicinal material Anoectochilus roxburghii with good results. And the recovery of dried Anoectochilus roxburghii was 88.9%~108.0%.

Key words:　Anion chromatograghy-Integral pulse ampere method; Anoectochilus roxburghii; free amino acids

E-mail: zth23-1980@163.com

Analysis and Testing Technology and Instruments, Vol. 17, No. 2, 2011,74-78