YAN Chao-ze1, ZHANG Shuai1, XIN Jia-ying1,2*, XIA Chun-gu2
(1. Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076,China; 2. State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000,China)
Abstract: Chrome azure S (CAS) and Cu ions form blue-purple complex under the existence of hexadecyl trimethyl ammonium bromide. EDTA and methanobactin(mb) may change the color of the CAS-Cu complex, respectively, because of the copper-binding ability. In the spectrophotometric method, the copper-binding curve of EDTA and methanobactin can be drawn, and the relative concentration of mb to EDTA can be calculated by a regression equation. Now a spectrophotometric method to detect methanobactin is designed, the maximum absorbance is 605 nm,and linear regression equation is Y=-3.5X+0.00536，and R2=0.9946, showing the linear relationship is good. The test results of practical samples show that this method is simple, sensitive, accurate and reproducible.
Key words: chrome azure S ( CAS ); methanobactin; EDTA;hexadecyl trimethyl ammonium bromide ( HDTMA )
E-mail: xinjiaying@yahoo.com.cn
Analysis and Testing Technology and Instruments, Vol. 17, No. 2, 2011,69-73
