Qiu Yue-qin, Chang Yin-xia, Du Li-ming*

( Analytical and Testing Center, Shanxi Normal University, Linfen 041004,China )

Abstract:The effect of 3,5-dimethylphenyl isocyanate on the chiral recognition of　 hydroxypropyl-β-cyclodextrin bonded stationary phase has been studied. Hydroxypropyl- β-cyclodextrin, bonded to 3-aminopropyltriethoxysilane through (3-isocyanatopropyl) triethoxysilane was used as the coupling agent, then other hydroxyl groupsofβ-CD and silica gel were derivatized with 3,5-dimethylphenyl isocyanate, thus a 3,5-dimethylphenylcarbamate perderivatized hydroxypropyl-β-cyclodextrin bonded silica gel stationary phase was prepared. The chiral resolution of nine chiral drugsin reversed phase chromatography showed that 3,5-dimethylphenylcarbamate perderivatized hydroxypropyl-β-cyclodextrin bonded stationary phase has better separation result than that of hydroxypropyl-β-cyclodextrin bonded stationary phase.

Key words: hydroxypropyl-β-cyclodextrin; chiral stationary phase; chiral separation

E-mail: lmd@dns.sxnu.edu.cn

Analysis and Testing Technology and Instruments, Vol. 17, No. 3, 2011,155-159