Liu Shao1,2 , Liu Hui-Lin1 , Dong Xin-Rong3* , Xie Da-Ping1
(1College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; 2 College of Food science and technology, Hunan Agricultural University, Changsha 410128, China; 3 College of Science, Hunan Agricultural University, Changsha 410128, China)
Abstract: The reaction kinetics for synthesis of capsinoids by lipase-catalyzed transesterification of vanillyl alcohol and methyl nonanoate in acetone was investigated in this work. The concentration of capsinoids obtained in the reaction was detected by HPLC and then the initial velocity of the synthesis reaction was calculated. The inhibiting test of product was carried out with methanol added. The results revealed that the enzymatic-synthesis reaction follows the Ping-Pang reaction mechanism.The kinetic equation with corresponding Vmax, KmA and KmB was inferred by the double reciprocal plot. The fitting degree of equation model arrives at 0.997 to compare with the experimental data.
Key words: capsinoids; Enzymatic-synthesis reaction; Lipase; Organic media; kinetics
E-mail: xinrong108@yahoo.com.cn
Journal of Molecular Catalysis, Vol. 25, Issue 3, 2011, 276~280
