Dong Li-feng1, Wang Yan1, Xin Jia-ying1, 2*, Lin-lin Chen1, Chun-gu Xia2
(1 Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin,150076; 2 State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000)
Abstract: Lipase-catalyzed synthesis of a new-type of antioxidant in solvent-free system was studied. Using Novozym435 as catalyst, transesterification of ethyl ferulate (EF) with different fatty alcohol to form fatty ferulate in solvent-free system was investigated. According to the yield of transesterification, ethyl ferulate (EF) with oleyl alcohol (OA) was chosen as the optimized substrate. The influences of reaction temperature, the ratio of enzyme to substrate, agitation speed, reaction time, water activity, the molar ratio of substrates, vacuum-rotary evaporation procedure and the stability of enzyme on the yield of oleyl ferulate were studied in detail.The process was optimized for the enzymatic synthesis of oleyl ferylate using a vacuum-rotary evaporation procedure under following conditions: 5.0 mmol oleyl alcohol, 1.0 mmol ethyl ferulate, 70 mg Novozym 435, water activity(aw)﹤0.01, at 60 ℃, 200 r/min agitation and 0.001 MPa for 96 h. The optimized conditions applied to the transesterification of ethyl ferulate with oleyl alcohol obtain oleyl ferulate yield of 65%. The enzyme can be reused several times without significant loss of activity.
Key word: ethyl ferulate; fatty alcohol; fatty ferulate; Novozym435 lipase; solvent-free system
E-mail: xinjiaying@yahoo.com.cn
Journal of Molecular Catalysis, Vol. 25, Issue 3, 2011, 262~268
