Abstract
In this work, deep eutectic solvents-based carbon dots (DESCDs) were prepared and bonded to the sil-ica surface for the first time to form a new hydrophobic chromatographic stationary phase (Sil-DESCDs). The successful preparation of DESCDs and Sil-DESCDs were demonstrated by a series of characterizations including transmission electron microscopies, laser scanning confocal microscope, Fourier transform in-frared spectrometry, elemental analysis, etc. Retention behavior of Sil-DESCDs was evaluated using Tanaka and Engelhardt standard test mixtures. The results showed that this new stationary phase had excel-lent separation performance for polycyclic aromatic hydrocarbons, flavonoids, aromatic amines and phe-nolic compounds. Excellent separation selectivity for the 3-phenylene ring isomers including phenan-threne and anthracene, the 4-phenylene ring isomers including pyrene, triphenylene, chrysene and 1,2-benzanthracene was also obtained. Especially, prednisolone and hydrocortisone, which have very similar structures, can be separated using pure water as the mobile phase. In addition, the flavonoids in As-tragalus extracts including calycosin-7-glucoside, ononin, calycosin and formononetin were determined using this new column, their concentrations were 0.050, 0.031, 0.023 and 0.034 mg/mL, respectively.(c) 2022 Elsevier B.V. All rights reserved.

Keywords Plus：COVALENT ORGANIC FRAMEWORKSQUANTUM DOTSPERFORMANCE

Published in JOURNAL OF CHROMATOGRAPHY A，Volume1681；10.1016/j.chroma.2022.463425，OCT 11 2022