Synthesized process of 4,4′-dipyridine modified silica stationary phase.
A novel multi-interaction stationary phase based on 4,4′-dipyridine modified silica has been synthesized and characterized by infrared spectra, X-ray photoelectron spectroscopy and elemental analysis by researchers from Key Laboratory of Chemistry of Northwest Plant Resources of the Chinese Academy of Sciences.
Mechanism involved in the chromatographic separation is the multi-interaction including π–π, hydrophobic, hydrogen-bonding, electrostatic and anion-exchange interactions. Based on these interactions, polycyclic aromatic hydrocarbons and phenols were successfully separated respectively in reversed-phase chromatography; inorganic and organic anions were also separated individually in anion-exchange chromatography by using the same column. Furthermore, the simultaneous separation of neutral organics, inorganic and organic anions was obtained on this stationary phase with the appropriate mobile phase. Therefore, such stationary phase has the characteristics of multi-interaction mechanism and multi-modal separation, and has potential application on complex samples.
The work has received support from the National Natural Science Foundation of China and the National Science & Technology Major Project of China.The findings have been published in Journal of Chromatography A (Journal of Chromatography A, 1218 (2011) 3743–3749).